Disulfide reduction

Moore; Borries Demeler; Senyon Choe.
PDA Journal of Pharmaceutical Science and Technology, issn.
Disulfide (S-S) bonds constitute one of the most important cross-linkages in proteins and have significant influence on the protein structure and function.
Some of the research activities that take place in our laboratories include but are not limited to:.Proteomic and genomic analyses to enhance our understanding of how the culture environment influences the protein production process.Kyle Zingaro; David (escc) Shaw; Jerry Carson; Anke Mayer-Bartschmid; Christian Bender; Christina Alves; Duncan McVey; Nan-Xin Qian; Qingxiang Wei; Mike Laird; Yuan Zhu; Robyn Emmins; John.For instance, NMR and crystallography require relatively large amounts (10 to 100 mg) of pure protein in a particular solution or crystalline state, can be limited by protein size, and are fundamentally low-throughput.For example, high salt concentrations hamper disulfide bond reduction, necessitating additional dilution of the sample with aqueous acidic solution at quench conditions.View on Scopus, genentech, Inc., Postdoctoral Fellow 1999, arizona State University, Microbiology,.Biotechnology Progress, issn: 87567938, peggy Ko; Shahram Misaghi; Zhilan Hu; Dejin Zhan; Joni Tsukuda; Mandy Yim; Mark Sanford; David (escc) Shaw; Masaru Shiratori; Brad Snedecor; Mike Laird; Amy Shen.Using computational fluid dynamics to understand how equipment differences influence mixing and mass transfer and using this information to optimize mixing conditions and control strategies.Investigating the relationships between productivity product quality attributes for both.Scientists our Scientists "We are responsible for developing clinical and commercial manufacturing processes for therapeutic proteins.".Standard, harvard, mysling, S, Salbo, R, code reduction colissimo 2016 Ploug, M Jørgensen, TJD 2014, 'Electrochemical reduction of disulfide-containing proteins for hydrogen/deuterium exchange monitored by mass spectrometry Analytical Chemistry, vol.
Our laboratories are housed within Late Stage Cell Culture as part of the Process Research Development organization.
We also identify some challenges in using electrochemical reduction in HDX-MS analyses and provide possible conditions to attenuate these limitations.
Vancouver, mysling S, Salbo R, Ploug M, Jørgensen TJD.
Major expression platforms include.Each of these frameworks has its advantages and disadvantages in terms of applicability, throughput, and accuracy.2014 Jan 7;86(1 340-5.Coli and Chinese Hamster Ovary (CHO) cells.Coli cultures at small- large-scale.Coli and CHO production systems.APA, mysling,., Salbo,., Ploug,., Jørgensen,.Implementation of plate imaging for demonstration of monoclonality in biologics manufacturing development.Pharmaceutical Biotechnology: Fundamentals and Applications, Fourth Edition, Le Dao; Barbara Lippe; Mike Laird.Blain; Witek Kwiatkowski; Qinghai Zhao; David La Fleur; Chethana Naik; Tae-Wook Chun; Tatiana Tsareva; Palanisamy Kanakaraj; Mike Laird; Rutul Shah; Lisa George; Indra Sanyal; Paul.We are responsible for developing clinical and commercial manufacturing processes for therapeutic proteins.The reduction typically requires a high concentration ( 200 mM) of the chemical reducing agent Tris(2-carboxyethyl)phosphine (tcep) as its reduction rate constant is decreased at low pH and temperature.Iowa State University, Biology,.S.

Our results demonstrate that efficient disulfide bond reduction is readily achieved by implementing an electrochemical cell into the HDX-MS workflow.
View Abstract on PubMed, human growth hormone.